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BIOLĀ 517. Laboratory in Human Embryonic Stem Cell Culture. 3 Units.

Prerequisites: BIOL 300 with a grade of "C" or better and consent of instructor
Advanced laboratory training in plating and passaging of human embryonic stem cells and human induced pluripotent stem cells. Mastery of a variety of cell culture techniques including isolation and culturing techniques including isolation and culturing of mouse embryonic fibroblasts, PCR and flow cytometry and immunohistochemistry on human embryonic stem cells and embryoid bodies. One hour of lecture 6 hours of lab. Materials fee required.